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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Cancer Prev Res (Phila). 2012 Apr 16;5(6):810–821. doi: 10.1158/1940-6207.CAPR-11-0532-T

FIGURE 1. Dysplastic and hyperplastic histology was progressively reversed over 14 days when TAg was downregulated at 4- but not 7-months of age.

FIGURE 1

Representative H&E sections illustrate phenotypic reversal of ductal cells in the submandibular salivary gland accompanying TAg downregulation in 4- (A) but not 7- (B) month-old Tet-op-TAgMMTV-tTA (tTa/TAg) mice over a 14-day timecourse. Arrows point to dysplastic ductal epithelial cells with nuclear pleomorphism and hyperchromasia and arrowheads point to striated ductal epithelial cells with normal nuclear appearance. The histological phenotype at both ages consists of varying percentages of hyperplastic and dysplastic (C), in transition (D), and normal-like (E) structures resembling those of wild-type mice (F). Stacked bar graphs shows distribution of hyperplastic and dysplastic, in transition and normal-like structures before and 14 days after TAg downregulation in 4- (G) and 7-month-old (H) mice. Timepoint (days): 0; prior to TAg downregulation, 2, 6, 10 and 14 indicate the number of days of doxycycline administration to downregulate TAg. m= age in months. Magnification H&E images = 60X. Size bar = 10 µm. *, **, ***:p<0.05, Student’s t test (two-tailed). Cohort sizes at different timepoints: 4m: 0(n = 4), 2(n = 4, 6(n = 4), 10(n = 4), and 14(n=5); 7m: 0(n = 3), 2(n = 3), 6(n = 3), 10(n=3), 14(n = 5).