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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biomaterials. 2012 May 20;33(24):5803–5811. doi: 10.1016/j.biomaterials.2012.04.051

Fig. 1.

Fig. 1

Schematic Representation of Chemistry Utilized to Append Recombinant CD47 Protein to Synthetic Biomaterials. Thiolated surfaces were provided by reacting the polymeric surface with a water soluble polymer, PDT-BzPh, photo-reacted, and reduced using TCEP as detailed in Materials and Methods. To append the recombinant CD47 poly-lysine protein, the protein was first reacted with SPDP to generate a thiol reactive site which then is able to bind to the activated synthetic biomaterials surface.