Fig. 3.

Scanning electron microscopy analysis of CD47 modified and control surfaces following ex vivo analysis. Human whole blood was perfused over unmodified, clinical grade blood conduits or CD47 modified, via biotinylated CD47 (CD47B) or lysine modified CD47 (CD47L), surfaces. (A) Representative image shows extensive platelet (P) attachment and spreading onto unmodified surfaces. Leukocytes (L) were also observed on unmodified surfaces. In contrast, only an occasional platelet or erythrocyte (E) was noted on CD47 modified surfaces. Bar equals 25μm. (B) Quantitative analysis shows significantly p < 0.05 more platelets were present on SEM fields from unmodified blood conduits compared to CD47 modified blood conduit surfaces. (C) SEM analysis shows complete removal of cells from the luminal surface of blood conduits following the detachment protocol detailed in Materials and Methods. All scale bars equal 25μm