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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Hum Immunol. 2012 Apr 19;73(6):673–676. doi: 10.1016/j.humimm.2012.03.005

Figure 2.

Figure 2

Batch 2 analysis of IL-4 and IFN-γ production by primary human lymphocytes stimulated with PMA/Ionomycin performed 1 week after Batch 1. When evaluating the entire lymphocyte population, the cells harboring the SNP genotype (SNP(CC), n = 5) produced significantly less IL-4 and IFN-γ than cells with the wild-type genotype (WT(TT), n = 7) (p = 0.01 and 0.02 respectively). CD3+ T-lymphocytes were stained in Batch 2 to allow for separate analysis of the CD3+ population. CD3+ lymphocytes with the SNP(CC) genotype produced significantly less IL-4 than cells with the WT(TT) genotype (p-value = 0.02), and produced less IFN-γ, which trended towards significance (p-value = 0.07). The amount of IL-4 and IFN-γ produced is presented as the Mean Fluorescence Intensity as determined by flow cytometry.