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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 31;1821(8):1068–1077. doi: 10.1016/j.bbalip.2011.12.007

Figure 3.

Figure 3

The role of Drs2-Cdc50 and other factors in the formation and trafficking of transport vesicles between the late Golgi and the early endosome. The flippase activity of Drs2 is proposed to generate membrane curvature at sites of vesicle budding at the TGN. Arf, recruited to these sites by ArfGEF, mediates the recruitment of AP-1 and clathrin to the newly forming vesicle. Vesicle formation is controlled by various factors that regulate Drs2 flippase activity, including phosphatidylinositol-4-phosphate (PI4P), Sac1 (PI4P phosphatase), and Kes1 (oxysterol binding protein). At the early endosome, retrieval of Drs2-Cdc50 and the exocytic SNARE Snc1 back to the TGN is mediated by Rcy1 (F-box protein), an effector of the Ypt31 Rab protein, a sorting nexin complex (Snx4/41/42), and the Arf cycle, in particular the ArfGAP Gcs1.