Table 1.
Human cell lines screened for mLEEK expression
| Cell line | Source | mLEEK | Method |
|---|---|---|---|
| A431 | Epidermoid carcinoma | + | RT–PCR; western |
| HCT116 | Colorectal carcinoma | + | Western |
| HEK293 | Kidney | + | Western |
| HT1080 | Fibrosarcoma | + | Western |
| MDA MB 468 | Breast adenocarcinoma | + | RT–PCR; western |
| U87MG | glioblastoma | + | RT–PCR; western |
Abbreviations: mLEEK, mini-LEEK; RT–PCR, reverse transcription–polymerase chain reaction.
Cell lines were cultured from frozen stocks. RNA was extracted and analyzed by RT–PCR as described in Figure 1. Lysates were prepared for analysis by western blotting with anti-mLEEK antibody. Positive expression of mLEEK is indicated with ‘+’.