Fig. 2.

Electrophoretic mobility shift and competition assays with nuclear extract of HEK293 cells transfected with pCMV-CDX1 and allelic variants of SNP rs9547970 in POSTN. 1 Labeled G probe + nuclear extract(Cdx1−); 2 labeled A probe + nuclear extract (Cdx1−); 3 labeled G probe; 4 labeled G probe + nuclear extract (Cdx1+); 5 labeled G probe + nuclear extract (Cdx1+) + unlabeled G probe; 6 labeled A probe; 7 labeled A probe + nuclear extract (Cdx1+); 8 labeled A probe + nuclear extract (Cdx1+) + unlabeled A probe. Cdx1−, from untreated HEK293; Cdx1+, from HEK293 transfected with pCMV-CDX1