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Global optimization of Vj gating of Cx45 homotypic (A and B) and Cx43-EGFP/Cx45 heterotypic (C and D) GJs. Experimental and simulated gj-Vj plots in A and C are shown in black and gray, respectively. (B and C) Open probabilities of fast and slow gates residing in hemichannels A and B (PF,A, PS,A and PF,B, PS,B, respectively) depending on Vj. P-Vj plots were simulated using values of parameters for fast and slow gates obtained during GCO.
