Figure S5.

Custom Opossum CTCF Antibody Design and Validation, Related to Experimental Procedures

(A) Alignment of parts of CTCF's protein sequences in multiple mammals. The peptides used to generate the commercial (human, rhesus, mouse, rat, dog) and custom (opossum) antibodies are highlighted.

(B) Wiggle tracks of CTCF and cohesin (STAG1/SA1) binding in opossum liver around the APP1 gene. The binding event highlighted with a star is in the orthologous location of the human binding event used for DNase I footprinting (Quitschke et al., 2000).

(C) Violin plots of raw read counts in opossum CTCF binding events for both replicates and cohesin validating that most opossum CTCF-binding events show strong cohesin enrichment.

(D) Scatter and Bland-Altman plots comparing the opossum CTCF to the opossum cohesin replicates. Spearman correlations are indicated.