Fig. 3.

Co-chromatography of FMRFamide immunoreactivity with synthetic Aea-HP-1. An acid–methanol extract of MAGs was fractionated using RP-HPLC. The elution of Aea-HP-1 was monitored using both MALDI/TOF-MS and ELISA for FMRF-amide-like peptides. Aea-HP-1 was detected by MALDI/TOF-MS (molecular ion, m/z 1227.6) in fractions 22–24 which corresponded to the retention time of synthetic Aea-HP-1. The same fractions were the only ones that gave an above background response in the ELISA. The calibration plot for Aea-HP-1 was linear between 10 and 1000 fmol.