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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Am J Primatol. 2012 Jul;74(7):676–686. doi: 10.1002/ajp.22022

Figure 2.

Figure 2

Spoligotype of M.tuberculosis H37Rv and M.bovis reference strains, two negative controls, and samples collected from macaques in Sulawesi. Spoligotyping is a strain differentiation method based on polymorphisms of a repeat sequence of the mycobacterial chromosome. Each mycobacterial strain varies in the number of repeats and the sequences between them, resulting in a distinct banding pattern. All five monkey sample spoligotypes indicate MTBC DNA, though the quantity of DNA available from the buccal swabs was too limited to provide complete spoligotype strain verification. However, comparison of the two reference strains with macaque samples in column 7 (lane b) and column 12 (lanes b and c) indicate that the samples are not M. tuberculosis H37Rv laboratory strain. Column 2 (lane c), circled in green, column 5 (lane a) and columns 14 and 15 (lanes a,b,c and a respectively), all circled in green indicate that the MTBC DNA in the Sulawesi pet samples is not M.bovis.