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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Immunol. 2012 Feb 1;188(5):2127–2135. doi: 10.4049/jimmunol.1102412

Figure 3. Vitamin D induces MKP-1 by human and mouse monocytes/macrophages.

Figure 3

Human PBMC were cultured in hormone-free medium containing 10 nM 1,25(OH)2D3 or vehicle control for 24 h. Adherent PBMC fraction was collected. (A, B) Human MKP-1 and MKP-5 mRNA levels were tested by real-time PCR and normalized to beta-actin mRNA levels (n=6 experiments). (C) Human MKP-1 protein levels were tested by Western blot. (D) Fold changes in the densitometry of human MKP-1 to beta-actin expression from Western blot are provided (n=4 experiments). (E) Murine BMM cells were cultured in DMEM for 18 h, and then treated with 10 nM 1,25(OH)2D3 or vehicle control for 6 h. mRNA levels of murine MKP-1 were tested and normalized to beta-actin mRNA (n=3 experiments). All values represent mean±SEM.