Figure 6. Inhibition of LPS-induced production of IL-6 and TNF-α in bone marrow macrophages from wild type mice by vitamin D was significantly compromised in MKP-1^−/− mice.

BMMs from wild-type and MKP1^−/− mice were cultured in DMEM containing 10 nM 1,25(OH)₂D₃ or 75nM (30ng/ml) 25(OH)D₃ for 24 h, followed by 10 ng/ml LPS stimulation for 24 h. IL-6 (A) and TNF-α (B) protein levels in the culture supernatants were detected by ELISA. Values represent mean±SEM (n=3 experiments).