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SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto Boyden chambers with an 8 μm pore membrane. The wells were treated with Y-27632 to study the impact of ROCK inhibition on cell invasion in a low- and high-density environment. A 3.5-fold increase in cell invasion was observed in Y-27632-treated cells seeded at a lower density of 50 × 103 cells/cm2.
