Figure 5.

Endocytosis of peroxidase following electrical stimulation. (A) Vesicle cluster in a neuron fixed during stimulation in the presence of external peroxidase. Large multivesicular bodies (mvb) are also present. Peroxidase was detected by a polyclonal antibody coupled to 10 nm gold particles. The black electron dense spots are associated with vesicles in the clusters located near the plasma membrane (pm), and labeling was considerably lower outside of the cluster. However, multivesicular bodies (arrows) and membranes of endoplasmic reticulum (arrowheads) also displayed gold particles. Scale = 1.0 μm. (B) Higher magnification of a vesicle cluster with electron dense spots accumulated over the vesicles. Scale = 200 nm. (C) Cluster with dense core vesicles and multivesicular bodies bearing gold particles. Scale = 1 μm. (D) Higher magnification of a multivesicular body that accumulated gold particles. Scale = 500 nm.