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. 1988 Jul 11;16(13):6015–6025. doi: 10.1093/nar/16.13.6015

Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.

L E Pyle 1, L N Corcoran 1, B G Cocks 1, A D Bergemann 1, J C Whitley 1, L R Finch 1
PMCID: PMC336844  PMID: 2840639

Abstract

The sizes of large DNA fragments produced from genomes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides, GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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