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. 1988 Jul 11;16(13):6097–6111. doi: 10.1093/nar/16.13.6097

Sequence and organization of barley yellow dwarf virus genomic RNA.

W A Miller 1, P M Waterhouse 1, W L Gerlach 1
PMCID: PMC336850  PMID: 3399386

Abstract

The nucleotide sequence of the genomic RNA of barley yellow dwarf virus, PAV serotype was determined, except for the 5'-terminal base, and its genome organization deduced. The 5,677 nucleotide genome contains five large open reading frames (ORFs). The genes for the coat protein (1) and the putative viral RNA-dependent RNA polymerase were identified. The latter shows a striking degree of similarity to that of carnation mottle virus (CarMV). By comparison with corona- and retrovirus RNAs, it is proposed that a translational frameshift is involved in expression of the polymerase. An ORF encoding an Mr 49,797 protein (50K ORF) may be translated by in-frame readthrough of the coat protein stop codon. The coat protein, an overlapping 17K ORF, and a 3'6.7K ORF are likely to be expressed via subgenomic mRNAs.

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Selected References

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