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. 2012 May 29;2012:548430. doi: 10.1155/2012/548430

Table 1.

Summary of the sequences of RT-PCR primers, the appropriate annealing temperature used in experiments, and product size.

Genes Primer Annealing temperature (°C) Product size (bp)
GAPDH S 5′-TCAACGGCACAGTCAAGG-3′ 54 299
A 5′-ACCAGTGGATGCAGGGAT-3′
RST S 5′-CACAGTGGGCAGACTGGACCAGAGC-3′ 57 412
A 5′-CCAAGGATGAGCGAAGGA-3′
OAT1 S 5′-TAATACCGAAGAGCCATACGA-3′ 56 358
A 5′-TCCTGCTGCTGTTGATTCTGC-3′
MRP4 S 5′-AAATCGGAATCTCCTGTCTG −3′ 56 203
A 5′-TATGAGGTCGGCGAATGA-3′
ABCG2 S 5′- TAGCAGCAAGGAAAGAC−3′ 54 835
A 5′-TGATGACAGAACGAGGTA-3′
XDH S 5′-CTTTGCGAAGGATGAGGTT-3′ 58 412
A 5′-CACTCGGACTACGATTCTGTT-3′
CPT1 S 5′-CCACGAAGCCCTCAAACAGA-3′ 57 315
A 5′-AGCACCTTCAGCGAGTAGCG-3′
OCTN2 S 5′-AGGTTTGGTCGCAAGAATG-3′ 56 458
A 5′-AACTCACTGGGATCGAAGAT-3′
PPARα S 5′-GGCTCGGAGGGCTCTGTCATC-3′ 56 655
A 5′-ACATGCACTGGCAGCAGTGGA-3′
SREBP-1 S 5′-GGAGCGAGCATTGAACTGTAT-3′ 58 344
A 5′-GGGCAGCCTTGAAGGAGTA-3′
PGC-1β S 5′-GGTACAGCTCATTCGCTACAT-3′ 58 210
A 5′-TAGGGCTTGCTAACATCACA-3′