Skip to main content
. Author manuscript; available in PMC: 2012 Jun 6.
Published in final edited form as: Science. 2011 May 13;332(6031):848–852. doi: 10.1126/science.1203919

Fig. 4.

Fig. 4

The Rasgrf1 direct repeat serves as a promoter for pit-RNA. (A) Detection of pit-RNA by reverse transcription polymerase chain reaction (RT-PCR) in germ cells from 16.5-dpc testes. Oct4 is a marker for germ cells. (B) Detection of a pit-RNA cleavage site by modified RACE. The numbers of cDNA ends obtained by sequencing the recovered RACE products are also shown (right). (C) Transcription start-site analysis by 5′ RACE. The number of cDNA ends determined by sequencing the RACE products is shown with an arrow in the schematic representation of the region (bottom). The positions of the PCR primers for the analyses are also illustrated. (D) Quantitative PCR analysis of pit-RNA in wild-type and MitoPLD−/− testes at 16.5 dpc. Error bars represent standard deviation (n = 3). The level of Oct4 mRNA was used as a reference. (E) Quantitative PCR analysis of pit-RNA in wild-type and direct repeat mutant testes at 16.5 dpc. Error bars represent standard deviation (n = 3). The level of Oct4 mRNA was used as a reference.