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. 2012 May 29;7:2661–2671. doi: 10.2147/IJN.S30445

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© 2012 Xu et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

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Confocal laser scanning microscopy of cellular incorporation and release of doxorubicin in A549 multicellular spheroids. (A1) A549 spheroid cells incubated in free doxorubicin for 4 hours. (A2) Free doxorubicin released from the spheroid for one hour. (B1) A549 spheroid cells incubated in composite doxorubicin-loaded micelles with the same doxorubicin densities for 4 hours. (B2) Composite doxorubicin-loaded micelles released from the spheroid for one hour. (B3) Composite doxorubicin-loaded micelles released from the spheroid for 2.5 hours.