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. Author manuscript; available in PMC: 2012 Jun 6.
Published in final edited form as: J Nutr Food Sci. 2011 Nov 24;Suppl 2:001.

Figure 1. Elimination of ROS and attenuation of endoplasmic reticulum (ER) stress by wolfberry phytochemicals in leukemia Jurkat cells.

Figure 1

Cells were treated with 0.5, 1, and 2 mg/ml wolfberry phytochemicals for 48 h. Phosphate buffered saline (PBS) treatment as a control. The cellular ROS levels were determined (A). The whole cell lysates were subjected to Western blot (B). Representative images were shown. Total pixels of each protein band were used for graphing and statistical analysis, based on at least three independent Western blot experiment results. *: significance at p ≤ 0.05. GAPDH as a loading control. ROS, reactive oxygen species; Grp78, glucose regulated protein 78; IRE1, inositol-requiring protein-1; ATF6, activating transcription factor -6; PERK, protein kinase RNA-like ER kinase; CHOP, C/EBP-homologous protein; IB, immunoblot; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.