Figure 3.

Microarray analysis of the effects of early postnatal UNO on olfactory bulb transcriptome of young adult mice. (a) Expression profile of 103 genes from the >20,000 total on the chip that met arbitrary significance criteria (2.25-fold up, or 2.25-fold down, with P < 0.01). Tissue source is shown on bottom axis. Note that there were three technical replicates within each of three biological replicates (subscript numbers). Color represents expression value with red, upregulation and green, downregulation. Dendrograms based on expression values show clustering genes (left) and samples (right), respectively. Note large number of up- and downregulated genes in both occluded and open (nonoccluded) bulb with normal bulb showing intermediate expression of most genes. (b) Volcano plot of 16,456 genes detected by the array for the comparison of occluded versus open olfactory bulb in UNO mice. Transcript abundance (log₂) is plotted on the abscissa. Statistical significance (log₁₀) is plotted on the ordinate. Genes shown in red meet a 2-fold and P < 0.01 criterion. Note that there are more upregulated genes (+) than downregulated genes (−) on occluded side.