Fig. 4.
MDCK cells transfected with mock (blue) and Sept2 siRNAs (red) for 18 hours. Scale bars, ~5 μm. (A to C) Cells were stained with DAPI and CREST, CENP-E, MCAK, and Mad2 antibodies. (D) Cells were incubated with 35 μM nocodazole (nzd) for 2 hours at 37°C. (E) Percentages of CENP-E–, MCAK-, and Mad2-positive kinetochores per cell. An average of 110 kinetochores were counted per cell (17); the number of cells counted is shown within each bar. Error bars represent the 95% confidence interval of the mean value according to the unpaired Student’s t test.