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. Author manuscript; available in PMC: 2012 Jun 6.
Published in final edited form as: Nat Cell Biol. 2005 May;7(5):463–473. doi: 10.1038/ncb1248

Figure 5.

Figure 5

Endogenous EB1 binds along the length of microtubules on basal patches of MDCK and Caco-2 cells. All scale bars, 5 μm. (a) Examples of immunofluorescence staining of EB1 (red) and microtubules (green) shows EB1 puncta along the length of microtubules on basal patches of MDCK cells. EB1 is present at intersection points between microtubules (arrowheads). EB1 often fills in discontinuities in microtubule staining (arrows). (b) Immunofluorescence of EB1 staining at the base of an intact MDCK cell shows the same distribution as on isolated basal membrane patches (compare with a). The boxed region in the right panel is enlarged in the left panel. (c) Co-staining of APC (green) and EB1 (red) on basal patches from MDCK cells shows the difference in protein distributions. APC puncta are localized to the membrane cortex in areas devoid of microtubules, and along the length of microtubules, but not in a pattern overlapping with EB1 puncta. (d) Another example of APC and EB1 co-staining on an MDCK basal patch, showing that APC is in a punctate distribution on microtubules and the membrane cortex, whereas EB1 is confined to microtubules in a smoother distribution. (e) Staining of EB1 on a Caco-2 cell basal patch shows that EB1 puncta are localized along the length of microtubules. (f) Two examples of APC (green) and EB1 (red) staining on Caco-2 cell basal patches show that these proteins do not co-align.