Figure 3.

FAUC 185 (5b) displays binding character on the D_2LF390^6.52W receptor undistinguishable from that on the D_2L wild-type receptor. Data obtained from [³H]spiperone displacement studies with FAUC 185 (5b) on membrane preparations from stably transfected CHO cells that expressed either wild-type D_2L or D_2L F390^6.52W receptor yielded biphasic curves. The fraction of high-affinity sites was 30% in D_2L wild-type and 29% in D_2L F390^6.52W receptor. The affinities were 1.1 nM (K_high) and 180 nM (K_low) for D_2L wild-type and 2.5 nM and 200 nM for D_2L F390^6.52W receptor. Normalized curves with error bars representing the SEM are shown.