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(A) Changes in absorbance of HLA20A and HLA20 (both 0.1 mM in 5%NH4Ac, pH = 6.9) on addition of metal salts (0.05 mM): (a) HLA20A; (b) HLA20A + ZnCl2; (c) HLA20; (d) HLA20 + ZnCl2; (e) HLA20−Zn complex + CuSO4. (B) Job plots for complexation of HLA20 with Zn2+ determined by UV−vis spectrophotometry in 5% NH4Ac (pH = 6.9) at room temperature, [HLA20] + [Zn2+] = 0.2 mM. Results are values of three assays.
