Skip to main content
. 2010 Mar 25;1(6):463–473. doi: 10.1021/cn100010p

Figure 2.

Figure 2

Clozapine, olanzapine, and trifluoperazine inhibit DAF-16::GFP nuclear localization within similar concentration ranges. (A−C) Dose−response for clozapine, olanzapine, and trifluoperazine suppression of DAF-16::GFP nuclear accumulation. In this and later figures, Nuclear DAF-16::GFP represents the ratio of cytoplasmic to nuclear fluorescence as a percent of the cytoplasmic/nuclear fluorescence of the no-drug controls as described in Methods (for each condition, n = 20; two tailed Student’s t-test, *P < 0.05, **P < 0.01 mean ± SEM). (D) Clozapine (160 μM), olanzapine (160 μM), and trifluoperazine (80 μM) have roughly the same effect on neurons, hypodermal cells, and gut cells in vivo. Neurons, hypodermal cells, and gut cells were measured for each condition as described in Methods (n = 200, two tailed Student’s t-test, *P < 0.05, **P < 0.01 mean ± SEM).