Figure 4.

Broad range of antipsychotic drugs increase signaling to DAF-16. Inhibition of DAF-16::GFP nuclear accumulation by a broad range of antipsychotic drugs that include all of the major classes of typical antipsychotics, all of the commonly prescribed atypical antipsychotics, and the muscarinic receptor agonist xanomeline, which represents a new class of antipsychotic drug. The concentrations chosen were in each case based on previous experiments. (Drugs marked by † were tested at 80 μM, all other drugs were tested at 160 μM.) Serotonin (5-HT, 2.5 mM) provided a positive control (for each condition n = 20; two tailed Student’s t-test, *P < 0.05, **P < 0.01 mean ± SEM).