Figure 1.

Isolated bead−axonal membrane complexes detach and readily adhere to the applied PDL-coated coverslip. (A−C) Representative image panel of a bead complex isolated from DIV14 neurons incubated with PDL beads for 24 h. The neuronal cultures were labeled with the membrane dye DiI for 1 h prior to patch preparation. Note the puncta-like shape of the DiI clusters along the bead surface (B, white arrowheads), suggestive of presynaptic-like endings. Scale bar, 5 μm.