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(i) Hippocampal neurons are first cultured on a coverslip for 7-15 DIV and then incubated with PDL-coated beads for 24 h. (ii) Following incubation, a second coverslip (pre-coated with PDL) is placed on top and brief pressure is applied. (iii) Careful removal of the top coverslip reveals beads to which isolated presynaptic compartments are attached.
