Table 5.

Primers used for the validation of gene expression and alternative splicing

Gene	Primer sequence 5'→3'	Amplicon size (bp)
Primers for gene expression validation
GBG5	F: TCCAGCGTCGCGGCTATGAA	94
	R: TCAAATCTGCAGCTGCCTGGGA
PGP	F: TGAAGCGGCTGGGCTTCCCTAT	131
	R: GGGCACCAACATGGACAACCGA
QOP5F6	F: TGGTGACGTGCACCCGAATGA	112
	R: AGCACCCACGATGGGGAAGATC
CFL1	F: TGGTGTCGACGACTTACGCACT	132
	R: AGCGCCTCTCGTCTTGTAGGCT
B4GALT4	F: TGATACCGGCCTCTGTGCACCT	113
	R: TGCCCTTCTGTGTCTCCACACCT
MYL7	F: ACCCCAGTGGTAAAGGCGTGGT	115
	R: TCCATGGGTGTCAGGGCGAACA
DUS6	F: GGGCGAACTCGGCTTGGAACTT	118
	R: AACGAGAATACGGGCGGCGA
Primers for alternative splicing validation
AP2B1-F1	GCCTAGATAGTCTGCTTGGCA	134
AP2B1-R	ACCACTGCTGACCACAGC
AP2B1-F2	GCCTAGATAGTCTGGTGGGA	92
AP2B1-R	ACCACTGCTGACCACAGC
ZDHHC16-F1	TGCTGCCATCGAGACTTA	97
ZDHHC16-R	GCACAGGAACCAGAGGTAGAC
ZDHHC16-F2	CGACAAGAACAAACTACAGG	118
ZDHHC16-R	GCACAGGAACCAGAGGTAGAC