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SDS-PAGE of HaloTag® fusion proteins incubated with HaloTag® Alexa Fluor 488 ligand. M refers to PageRuler Plus prestained protein ladder (Fermentas) with fluorescent bands at 70 kDa and 25 kDa, The HaloTag® standard protein (HT-SP) was added as an additional size reference at 60 kDa. The bands match the expected sizes for each fusion protein. Additionally small fragments of only HaloTag® (34 kDa) are visible which might be due to early-terminated translation.
