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. 2012 Jun 6;7(6):e38514. doi: 10.1371/journal.pone.0038514

Figure 4. Release of internal vesicles.

Figure 4

(A) Following collapse of an L-form mother cell, intact progeny cells are released. Membranes are visible by using DIC (Differential Interference Contrast) microscopy (left); dye-labeled membranes (right) are shown immediately before (upper) and after the collapse (lower). (B) A vesiculated cell labeled with Rho123 (upper) disintegrates and releases a vesicle (lower image, arrow head). The increase in the Rho123 signal on the surface of the released vesicle suggests an increase in membrane charge.