Figure 2. Effects of miR-145 on inhibiting angiogenesis and invasion in vitro. (A) Tube formation assay of HUVEC. A significant decrease was observed in the cells transfected with miR-145 precursor compared with the cells transfected with scrambled control (miR-scr). *p < 0.05. (B) Matrigel invasion assay of MCF7 and MDA-MB-231 cells transfected with miR-scr or miR-145 (×200). A significant decrease was observed in miR-145-transfected cells. *p < 0.05. **p < 0.01. (C) Cell viability was tested 72 h after transfection by trypan blue staining. (D) Immunoblotting analysis of genes involved in IRS1-N-RAS-VEGF pathway in breast cancer cell lines using antibodies against IRS1, N-RAS, PIK3CA, PIK3R1, AKT, p-AKT, mTOR, p70S6K1 and VEGFA. β actin level served as an internal control.