Figure 5. Forced expression of N-RAS rescues miR-145-inhibited tumor growth in vivo. (A and B) Tumor volumes (A) and weight (B) induced by MDA-MB-231 cells expressing lenti-scr and lenti-miR-145 with GFP or N-RAS were analyzed and presented as mean ± SD, n = 5. (C) Immunohistological analysis of microvessels in tumor sections using Factor VIII (×40). Overexpression of miR-145 (middle) inhibited microvessels formation when compared with the scrambled control (left); while re-expression of N-RAS (right) reversed microvessels formation suppressed by miR-145. (D) Quantitative MVD analysis (upper) and hemoglobin contents (lower) were analyzed using above tumors.