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. 2012 Jun 6;7(6):e38735. doi: 10.1371/journal.pone.0038735

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(A) Reverse phase (C18) chromatography of insect cadaver extract. The image represents two independent experiments. The estimated physiologically relevant concentration was used in the assays; Frc, fraction. (B) Testing physiologically relevant concentration of ascarosides found in Frc A (ascr#9, 40.3 pmol/µl and ascr#11, 1.3 pmol/µl). Image represents three experiments. (C) Ascr#2 and ascr#9 are structural analogs. Natural and synthetic ascr#9 were tested in combination with fraction B and C. Image represents four experiments. (D) Structure of ascr#11. It (1.3 pmol/µl) is also sufficient to cause dispersal in combination with fractions B and C. Image represents three experiments.