Figure 4. BCR-FGFR1 derived cell lines are consistent with an pro-B cell phenotype.

(A) Western blot analysis shows the presence of the 120 kD BCR-FGFR1 fusion protein in BBC1 and BBC2 cells compared with normal thymocytes. (B) Genomic PCR analysis of IgH rearrangement showing the germline configuration in the tail and polyclonal rearrangements in the spleen from normal mice. (C) Flow analysis of BBC1 and BBC2 cell lines demonstrates an immature B-cell immunophenotype.