Table 4. Primers of interest and reference genes used for quantitative real-time PCR assay.

Gene	Full name	NCBI accession number		Primer	Primer position
CycA	Cyclophilin A	NM_008907.1	Forward	5′-TGCTGGACCAAACACAAACG-3′
			Reverse	5′-GCCTTCTTTCACCTTCCCAAA-3′
Fabp7	Fatty acid binding protein 7	NM_021272.3	Forward	5′-TAAGTCTGTGGTTCGGTTGG-3′	329–348
			Reverse	5′-CCCAAAGGTAAGAGTCACGAC-3′	429–449
Gal	Galanin	NM_010253.3	Forward	5′-GAGAGCAACATTGTCCGCAC-3′	396–415
			Reverse	5′-ATGGTCTCAGGACTTCTCTAGG-3′	505–527
Glul	Glutamate-ammonia ligase	NM_008131.3	Forward	5′-TGAGAGAACCATCCTATTCACTG-3′	1927–1949
			Reverse	5′-TAAGCAGTAATGAAGCTGAGACC-3′	2045–2067
Igfbp3	Insulin-like growth factor binding protein 3	NM_008343.2	Forward	5′-GGAAACCCATAACCGAGTGAC-3′	1475–1495
			Reverse	5′-TCCCACAGTTCCCAAGTAGATC-3′	1585–1606
Npy1r	Neuropeptide Y receptor 1	NM_010934.4	Forward	GCGACTCAGAGCATTTCTAAC	2547–2567
			Reverse	GCAGTGAAGGATAAATGGTGAG	2637–2658
Sstr1	Somatostatin receptor 1	NM_009216.3	Forward	5′-GCTGTCCAATTGAGTATGCTGC-3′	3419–3440
			Reverse	5′-CAGGTCAGTGTGAACTTGCG-3′	3527–3546
Ywhaz	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide	NM_011740.3	Forward	5′-TCCTTATTCCCTCTTGGCAG-3′	2432–2451
			Reverse	5′-ATGGAAGCTACATTAGCGGTTT-3′	2502–2523

^*CycA primer sequences were described in published literature [74].