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. 2012 Jun 6;7(6):e38101. doi: 10.1371/journal.pone.0038101

Figure 1. Cerebellar granule neurons are robustly inhibited by CSPGs and partially rescued by Gö6976.

Figure 1

Dissociated postnatal cerebellar granule neurons (CGNs) were challenged in an inhibitory assay. A, B, representative images of CGNs transfected with control plasmid pSport mCherry growing on CSPGs (A), and a permissive substrate, laminin (B). C,D, the addition of the PKC inhibitor Gö6976 partially relieved CSPG inhibition (C), and potentiated laminin growth (D). E , Bar chart depicting ratios of seven parameters on CSPGs divided by laminin (with 95% confidence intervals). Growth on CSPGs led to large decreases in neurite length, the number of primary neurites, and the percentage of cells with neurites. Tubulin intensity and soma area were slightly increased. The neuron count was decreased on CSPGs, implying a deficiency in cell adhesion or survival. F, Percent increase by Gö6976 was plotted with 95% confidence intervals on CSPGs (open bars), and on laminin (solid bars). Gö6976 had a strong positive effect on neurons growing on CSPGs, especially for neurite length and the percentage of neurons growing neurites. Neuron count and viability was unchanged. Statistics with un-normalized treatment averages, Mann-Whitney Test, *p< = 0.05, **p< = 0.01, ***p< = 0.0001. Scale bar 100 µm.