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. 2012 Jun 6;7(6):e38101. doi: 10.1371/journal.pone.0038101

Figure 4. Hippocampal neurons are inhibited by CSPGs, and rescued by GPX3 overexpression.

Figure 4

Hippocampal neurons transfected with active genes were plated on CSPG or laminin substrates. A, CSPGs (white) strongly inhibited hippocampal growth compared to laminin (black) (p<0.0001, Mann Whitney U Test) in three independent experiments (triangle, square, and circle markers). Horizontal bar indicates the average neurite total length on CSPGs and laminin, 15.5 µm and 60.9 µm respectively. B,C Mean Z-Scores of transfected neurons with standard deviations, centered on the negative control, mCherry. Asterisks indicate significant effects (*, **, ***, p<0.05, 0.01, 0.001 Tukey-Kramer) compared to the mCherry control when analyzed with ANOVA (p<0.001). D, E, Representative images of hippocampal neurons growing on CSPGs transfected with GPX3 (D) or control mCherry (E). Scale bar 100 µm.