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. 2012 Jun 6;7(6):e38101. doi: 10.1371/journal.pone.0038101

Figure 5. GPX3 and GPX7 significantly increase neurite length of postnatal CGNs plated on the inhibitory MAG substrate.

Figure 5

Postnatal day 8 rat CGNs were co-transfected with the pmaxGFP plasmid (green) and the pCMVSPORT6 plasmid expressing either GPX3, GPX7, OGFLR1 or the control gene mCherry and plated onto a feeder layer of CHO cells expressing a non-inhibitory construct (R2), or the CNS myelin component, MAG. A. CGNs growing on CHO-R2 transfected with GFP and mCherry. B. CHO-MAG strongly inhibited the neurite outgrowth of CGNs transfected with GFP and mCherry. C. CGN neurite outgrowth is partially rescued when transfected with GPX3. D. Data are plotted as mean +/− SEM of 8 experiments, (One-way ANOVA, Dunnett’s post hoc, *p<0.05, ***p<0.001). Red channel marks β-tubulin positive neurons, green channel represents GFP expression, transfected neurons therefore appear yellow.