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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neurooncol. 2012 Mar 2;108(3):395–402. doi: 10.1007/s11060-012-0842-3

Fig. 5.

Fig. 5

Western blot and RT-PCR analysis reveals EGFRvIII expression in the diffuse pontine glioma neurospheres and tumor samples: a Western blot analysis showed the presence of EGFRvIII in DIPG neurospheres, tumor lysate and U87MG-vIII (positive control) but not in the U87MG cell lysate. b RT-PCR was performed on total RNA from DIPG neurospheres and tumor samples. Water and U87MG cells were used as a negative control and total RNA from U87MG-EGFRvIII cells were used as a positive control. EGFRvIII was detected in the tumor and neurosphere samples as well as the positive control but not the negative control