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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2012 Jan 8;131(3-5):83–92. doi: 10.1016/j.jsbmb.2011.12.005

Figure 1.

Figure 1

Generation of Tamoxifen and Aromatase inhibitor resistant cell lines. AI and Tamoxifen responsive cells were generated by engineering MCF-7 cells to overexpress aromatase. MCF-7aro cells were cultured long-term in the presence of tamoxifen or in the absence of estrogen until cells gained the ability to proliferate in these conditions. These cells are considered cells which have acquired either tamoxifen resistance (Tamoxifen-R) or AI resistance (LTEDaro). Anastrozole resistant (Anastrozole-R), Letrozole resistant (Letrozole-R), and Exemestane resistant (Exemestane-R) cells that have acquired resistance to a specific AI were generated by culturing MCF-7aro cells in the presence of testosterone and the appropriate inhibitor until cells gained the ability to proliferate in these conditions. Finally, models for de novo AI resistance were generated by overexpressing aromatase and HER2 or Akt.