Panel markers: 4-hydroxy-2-nonenal Michael adducts (4-HNE) (panels A, B, and C) and 8-hydroxy-2-deoxy guanosine (8-OHdG) (panel D, E, and F) in the renal cortex of the female mRen2.Lewis rat. The intensity of 4-HNE staining was quantified by Adobe Photoshop. Data are mean ± SE; *p<0.05 vs. HS; n=3-4. The number of 8-OHdG-positive nuclei in the renal cortex was counted with the automated counting software (Image Pro 6.3; MadiaCybernatics, Bethesda, MD, USA). Data are mean ± SE; *p<0.05 vs. HS group; n=4 per group.