Fig. 3.

Metallation state of MnSOD. Metal content of protein samples was measured before (a) and after (b) Mn reconstitution as described in the Methods for MnSOD from cultures grown at different temperatures: mitochondrial rhMnSOD isolated from yeast grown at 25, 30 and 37 °C; mitochondrial yMnSOD isolated from yeast grown at 30 °C; and yMnSOD isolated from E. coli grown at 37 °C in medium supplemented with MnCl₂ (*).