Table 3.
Metal Content and SOD Activity of recombinant MnSOD.
| Protein | T (°C)c | Protein Property | Before Mn reconstitution | After Mn reconstitution | Ref. |
|---|---|---|---|---|---|
| hMnSODa | 25 | Mn/monomer | 0.07 ± 0.01 | 0.70 ± 0.06 | This work |
| Fe/monomer | 0.15 ± 0.01 | 0.11 ± 0.02 | |||
| SOD activity (U/mg) | 925 ± 25 | 7115 ± 566 | |||
| 30 | Mn/monomer | 0.17 ± 0.01 | 0.71 ± 0.02 | This work | |
| Fe/monomer | 0.27 ± 0.05 | 0.13 ± 0.02 | |||
| SOD activity (U/mg) | 1406 ± 18 | 6639 ± 107 | |||
| 37 | Mn/monomer | 0.38 ± 0.01 | 0.71 ± 0.02 | This work | |
| Fe/monomer | 0.21 ± 0.03 | 0.15 ± 0.01 | |||
| SOD activity (U/mg) | 4734 ± 193 | 6900 ± 63 | |||
| hMnSOD | 37 | Mn/monomer | 0.84 ± 0.06 | n. d.d | 13 |
| Fe/monomer | n. d.c | n. d.d | |||
| SOD activity (U/mg) | 7164 ± 48 | n. d.d | |||
| yMnSODa | 30 | Mn/monomer | 0.87 ± 0.04 | n. d.d | This work |
| Fe/monomer | 0.20 ± 0.01 | n. d.d | |||
| SOD activity (U/mg) | 3823 ± 34 | n. d.d | |||
| yMnSODb | 37 | Mn/monomer | 0.83 ± 0.02 | n. d.d | This work |
| Fe/monomer | 0.03 ± 0.02 | n. d.d | |||
| SOD activity (U/mg) | 4353 ± 38 | n. d.d |
a
mitochondrial MnSOD isolated from yeast grown at different temperatures.
b
recombinant yMnSOD purified from E. coli grown at 37 °C in Fe-limited/Mn-supplemented media.
c
growth temperature.
d
n. d., not determined.