Western blot for ΔNp63α following 24 hours of contact lens wear. Proteins were resolved on a 7.5% SDS gel. (A–B) Wear of a non-oxygen transmissible PMMA lens significantly reduced ΔNp63α expression in the central corneal epithelium (A, p=0.025, t-test, n=3); and the limbal epithelium (B, p=0.009, t-test, n=3). (C–D) In contrast, ΔNp63α expression was not reduced following wear of a hyper oxygen transmissible lens (MZ) in the central corneal epithelium (C, p=0.005, t-test, n=3); or the limbal epithelium (D, p=0.004, t-test, n=3). β–actin was used as a loading control for all experiments. CTL: contact lens; (+) with lens; (−) no lens.