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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Eye Contact Lens. 2012 Jul;38(4):214–221. doi: 10.1097/ICL.0b013e3182482948

Figure 3.

Figure 3

Effects of hypoxia on ΔNp63α expression in hTCEpi cells in vitro. (A) There was no effect on ΔNp63α expression after 6 hours of treatment with CoCl2 compared to non-treated cells (p=0.495, t-test, n=3). (B) In contrast, a significant decrease in ΔNp63α was seen after 24 hours of treatment with CoCl2 (p<0.001, t-test, n=3). (C) A smaller, but significant reduction was seen in ΔNp63α following culture in 10% hypoxia for 24 hours (p=0.005, t-test, n=3); the decrease was more evident following 24 hour culture in 0% hypoxia (p=0.029, t-test, n=4).β–actin was used as a loading control for all experiments. (+) treatment group; (−) control.