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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Eye Contact Lens. 2012 Jul;38(4):214–221. doi: 10.1097/ICL.0b013e3182482948

Figure 4.

Figure 4

ΔNp63 transcriptional activity. (A&B) Luciferase activity after treatment with CoCl2 for the indicated time points compared to parallel control cultures. (A) Treatment with CoCl2 for 6 hours resulted in a significant increase in ΔNp63 activity compared to the non-treated control (p=0.013, t-test, n=4). (B) Treatment with CoCl2 for 24 hours also showed a corresponding increase in ΔNp63 activity compared to the non-treated control (p=0.029, t-test, n=3). Data was normalized to Renilla expression to control for variations in transfection efficiency. Due to the potential for variation in promoter activity under different culture conditions, data was then normalized to the pGL3 empty vector control. Non-CoCl2 treated controls were set at 1.