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The Probe Design Pipeline was composed of five main steps: 1 Selection of most representative SAS, 2 Identification of Orientation, coding and non-coding SAS and Selection by Functional Category, 3 Design of all available sense and antisense probes for selected SAS, 4 Exclusion of probes that do not agree with the criteria and 5 Probe Blast alignment against Sugarcane EST database to identify the unique probes
